Prep HPLC Column, Diamond Hydride, 4 um, 100 A. 21.2 mm ID x 50 mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑P21‑50 Diamond Hydride Preparative HPLC Column is engineered for high‑throughput purification workflows where sample load, robustness, and productivity outweigh maximum chromatographic resolution. This short‑bed preparative column format is optimized for rapid cycle times and efficient fraction collection when full‑length prep columns are unnecessary.
Preparative Use Case: Why 21.2 mm ID × 50 mm
The 21.2 mm internal diameter defines this column as fully preparative, intended for laboratories processing large sample loads and collecting milligram‑ to gram‑scale quantities of purified material.
The 50 mm column length offers distinct advantages for certain prep workflows:
Short residence time for fast separations
Lower backpressure at high flow rates
Increased sample throughput per day
Efficient purification when analytes are well resolved
This configuration is commonly selected when throughput, speed, and solvent efficiency are prioritized over maximum resolving power, and when longer 100–250 mm preparative columns are unnecessary.
Diamond Hydride™ TYPE‑C Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, Diamond Hydride stationary phases offer key advantages for preparative HILIC and mixed‑mode separations:
Highly reproducible retention of polar analytes
Faster equilibration compared to conventional bonded‑silica HILIC phases
Exceptional stability under high‑organic mobile‑phase conditions
Extended column lifetime in demanding preparative environments
This stationary phase is particularly effective for purification of:
Peptides and peptide‑like compounds
Metabolites and highly polar small molecules
Basic and amine‑containing analytes
Compounds best isolated via HILIC‑based mechanisms
Purpose‑Built for High‑Throughput Prep Work
The 70000‑P21‑50 enables predictable scale‑up from analytical or semi‑preparative HILIC methods while maintaining stable chromatographic behavior. It is often used in environments where speed, robustness, and sample throughput are paramount, including research, process development, and regulated purification workflows.
System Requirements
Typical operating flow rates: ~20–50 mL/min
This column requires a preparative HPLC system capable of:
Sustained high‑flow solvent delivery
Large injection volumes
Pressure compatibility with preparative hardware
Appropriate detector configuration and fraction collection
Standard analytical or semi‑prep HPLC systems are not suitable for 21.2 mm ID preparative columns.
Actual flow rates should be optimized based on solvent composition, viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
These columns represent a significant investment and are often application‑specific. To ensure customers receive the correct column for their workflow:
Pricing and technical support are provided through our Customer Service Department via formal quotation
Columns are produced on order, based on customer requirements
Orders cannot be canceled or returned once placed
Learn more about Custom Orders and our Custom Order policies.